Scatterplots are created using all of the following parameters EXCEPT which one?

Scatterplots are graphical representations that display the relationship between two variables. In the context of laboratory data analysis, particularly in hematology, scatterplots are often used to visualize the distribution of cell populations based on specific characteristics.

When considering the parameters used to create scatterplots, scatter, volume, and conductivity are all relevant. Scatter refers to the light scatter detected from cells as they pass through a flow cytometer, providing information on cell size and granularity. Volume represents the absolute size measurement of a cell, which can also be depicted in scatterplots to show variations in cell populations. Conductivity is a measurement related to the internal characteristics of the cells, reflecting their physical and chemical composition.

Fluorescence, while crucial in flow cytometry for identifying specific cell markers or components using labeled antibodies, does not directly influence the scatterplot construction, which focuses primarily on light scatter and size parameters. Thus, it is not a parameter used to create traditional scatterplots that represent relationships between cell populations in the context of hematology.