Which hemoglobins are differentiated by the Kleihauer-Betke technique?

The Kleihauer-Betke test is a specialized laboratory technique used primarily to differentiate between fetal hemoglobin (Hb-F) and adult hemoglobin (Hb-A). This technique is particularly useful in situations involving maternal-fetal hemorrhage, where it is critical to ascertain the presence and amount of fetal blood in the maternal circulation.

In the context of the provided choice, the distinction between Hb-A1 and Hb-F is key. Hb-F has a higher affinity for oxygen compared to Hb-A, which is why it remains stable through the Kleihauer-Betke staining process, enabling the differentiation between fetal and adult hemoglobin types. This capability is crucial for assessing cases of potential bleeding complications during pregnancy and ensuring proper maternal and fetal care.

The other options do not pertain to the Kleihauer-Betke technique, which is specifically designed for the identification of the fetal hemoglobin type in relation to adult hemoglobin. Therefore, the correct identification of Hb-F from Hb-A1 through the Kleihauer-Betke method illustrates the technique's specialized application in hematology.