Which of the following factors is NOT involved in the degree of packing during centrifugation?

The degree of packing during centrifugation is influenced by factors such as time of centrifugation, radius of centrifugation, and speed of centrifugation.

The time of centrifugation affects how long the particles have to sediment and become packed at the bottom of the centrifuge tube. Longer centrifugation times generally allow for more thorough packing of the samples.

The radius of centrifugation relates to the distance from the center of the rotor to where the samples are located. A larger radius means that the centrifugal force acting on the particles is greater, resulting in more efficient sedimentation and packing.

Speed of centrifugation is crucial as it determines the centrifugal force applied to the samples. Higher speeds lead to increased forces, which enhance particle sedimentation and packing density.

Temperature of centrifugation does not directly influence the degree of packing. While temperature can affect the viscosity of the medium and potentially impact the flow properties, the fundamental mechanisms of sedimentation reliant on mechanical forces of rotation, such as time, radius, and speed, are unaffected by temperature. Therefore, it does not contribute to the packing of particles during the centrifugation process.